![]() ![]() The PEGylated polyplexes had a significantly wider distribution in the tissue than the nonPEGylated polyplexes. PEGylated polyplexes also distributed more broadly among dermal fibroblasts and allowed greater interaction with antigen-presenting cells (APCs) (dendritic cells and macrophages) starting at around 24 h post-injection. By day 4, co-localization of polyplexes with APCs was observed at the injection site regardless of polymer structure, whereas small amounts of polyplexes were found in the draining lymph nodes. These in vivo findings demonstrate the superior stability of PEGylated polyplexes in physiological milieu and provide important insight on how cationic polymers could be optimized for DNA vaccine delivery. Khatri, Kapil Goyal, Amit K Gupta, Prem N Mishra, Neeraj Vyas, Suresh P Plasmid DNA loaded chitosan nanoparticles for nasal mucosal immunization against hepatitis B. This work investigates the preparation and in vivo efficacy of plasmid DNA loaded chitosan nanoparticles for nasal mucosal immunization against hepatitis B. Chitosan p DNA nanoparticles were prepared using a complex coacervation process. Prepared nanoparticles were characterized for size, shape, surface charge, plasmid loading and ability of nanoparticles to protect DNA against nuclease digestion and for their transfection efficacy. Nasal administration of nanoparticles resulted in serum anti-HBsAg titre that was less compared to that elicited by naked DNA and alum adsorbed HBsAg, but the mice were seroprotective within 2 weeks and the immunoglobulin level was above the clinically protective level. However, intramuscular administration of naked DNA and alum adsorbed HBsAg did not elicit sIgA titre in mucosal secretions that was induced by nasal immunization with chitosan nanoparticles. Similarly, cellular responses (cytokine levels) were poor in case of alum adsorbed HBsAg. Chitosan nanoparticles thus produced humoral (both systemic and mucosal) and cellular immune responses upon nasal administration. The study signifies the potential of chitosan nanoparticles as DNA vaccine carrier and adjuvant for effective immunization through non-invasive nasal route. Müller, J-M V Wissemann, J Meli, M L Dasen, G Lutz, H Heinzerling, L Feige, K In vivo induction of interferon gamma expression in grey horses with metastatic melanoma resulting from direct injection of plasmid DNA coding for equine interleukin 12. Whole blood pharmacokinetics of intratumourally injected naked plasmid DNA coding for equine Interleukin 12 (IL-12) was assessed as a means of in vivo gene transfer in the treatment of melanoma in grey horses. The expression of induced interferon gamma (IFN-g) was evaluated in order to determine the pharmacodynamic properties of in vivo gene transduction. Seven grey horses bearing melanoma were injected intratumourally with 250 µg naked plasmid DNA coding for IL-12. Peripheral blood and biopsies from the injection site were taken at 13 time points until day 14 post injection (p.i.). Samples were analysed using quantitative real-time PCR. I used bandaids.Plasmid DNA was quantified in blood samples and mRNA expression for IFN-g in tissue samples. One of the female teachers suggested I wear a bra to hide the nipples because it was distracting the boys. But just plain walking was not a problem. Had to go a bit easy on the stairs though. They thought they were hot stuff when I didn't have any but when they did come in, I was probably a 34D by age 16 and I could skip the bra if I wanted. soon as they had any size to them they started drooping. The city girls who had life a lot easier had more body fat and they started sprouting at about 12-13. When my boobies finally showed up between14-15, they came in fast and looked like cones, I kid you not. ![]() I grew up on farm and ranch, worked hard everyday. Straight out, pointing everywhere they are headed. Believe it or not, a girl with low body fat index will sprout breasts that are shaped like cones. so, they didn't have a lot of fat in their diet. Round attention Svg Vector Icons : Reportīack then, girls had to do a lot of physical work.Upvotes Follow Unfollow 3 years ago Dots Created by potrace 1.15, written by Peter Selinger 2001-2017 ![]()
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